Quality Assurance and Quality Control (QA/QC)

It is important that quality assurance/quality control (QA/QC) procedures are followed during sample collection and during training exercises by participating national officials. Important QA/QC considerations are described below:

  • Disposable latex gloves must be used to handle all samples and specimens, and should be changed between samples and/or specimens;
  • Stainless steel dissection trays and tools (scalpels, forceps, calipers, etc.) must be rinsed in distilled water, then acetone and hexane, before each use and between sample collections;
  • Sample jars must be pre-cleaned by the analytical laboratory prior to shipment to participating countries;
  • Tools and gloved fingertips which touch the skin or external organs of sampled animals should not touch internal tissues; and any tissue suspected of being contaminated in this manner must be discarded;
  • Multiple samples should be collected at all sampling stations. A subset of these samples will undergo more than a single assessment methodology. Results of different assessment methodologies will be compared;
  • All samples should be placed in 125 ml heat-treated, wide-mouth glass jars and sealed with lids lined with aluminum foil. Samples must be appropriately labeled, stored in a cool/dark area, and if they contain tissue, should be transported to freezer facilities within 2 hours of sample collection;
  • The location of each sampling station must be carefully recorded using a hand-held GPS, and photos/video to ensure repeatability of future sampling programs;
  • Detailed records must be kept of the owners of the local farms, fields, fish ponds and animals sampled. Interviews with local residents provide essential data on the source of animals sampled (i.e., sex, age, origin);
  • Bird or fish tissues should be sampled immediately after being slaughtered on-site (i.e., samples should not be purchased from the market unless the location of where they were raised can be verified); and
  • Smoking is not permitted in the vicinity of sampling activities.

Examples of Field QA/QC procedures

  Disposable latex gloves must be used to handle all samples and specimens, and should be changed between samples and specimens. Rinse gloves with distilled water before touching sampling equipment.
  Stainless steel dissection trays and tools (scalpels, forceps, shovels, etc.) must be rinsed in distilled water, then acetone and hexane, before each use and between sample collections.

 

All samples will be placed in 125 mL heat-treated, wide-mouth glass jars and sealed with lids lined with heat-treated aluminum foil. Samples will be appropriately labeled, wrapped with clear plastic tape and stored in a cool/dark area.

  The location of each sampling station must be carefully recorded using a hand-held GPS, and photos/video to ensure repeatability of future sampling programs. An additional GPS training manual is provided (see example field data sheets).

Next Steps

learn about Field Sampling Organization

Always use new latex gloves to avoid contamination
Source: Hatfield Consultants
Hatfield Consultants The World Bank funded by the Canadian POPs Trust Fund through the      
Canadian International Development Agency
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